G5154

Glasgow Minimum Essential Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): BHK medium, GMEM
• MDL number: MFCD00243118
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; phenol red: 0.0213 g/L; NaHCO₃: 2.75 g/L; glucose: 4.5 g/L (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

For use with adherent kidney cell lines such as baby hamster kidney cells (BHK).

Glasgow Minimum Essential Medium has been developed by modifying the Eagle′s BME medium. This medium can be used to study the genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used to culture:

• Hoxb7- green fluorescence protein (GFP) mouse embryonic stem (ES) cell line for the induction of ureteric bud differentiation
• murine E14 Tg2a embryonic stem (mES) cells
• transduced Chinese hamster ovary (CHO) cells for the expression and production of Siglec-1-Fc fusion proteins

Packaging

500, 500 mL

Reconstitution

Supplement with 0.292 g/L L-glutamine and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1