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G5160

Sigma-Aldrich

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

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Synonym(s):
β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

form

solid

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

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This Item
G5635G3153G6008
specific activity

≥8.0 units/mg solid

specific activity

≥500 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥250 units/mg protein

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

contains

dextrin as extender

contains

-

contains

-

contains

-

General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Hydrolysis of whey lactose by immobilized β-Galactosidase
Marcela Panaro Mariotti; Hideko Yamanaka; Angela Regina Araujo; Henrique Celso Trevisan
Brazilian Archives of Biology and Technology, 51 (2008)
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C M Seubert et al.
Cancer gene therapy, 18(1), 42-52 (2010-09-11)
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Martin Dragosits et al.
Applied microbiology and biotechnology, 98(8), 3553-3567 (2013-09-17)
Galactosidases are widespread enzymes that are used for manifold applications, including production of prebiotics, biosynthesis of different transgalactosylated products, improving lactose tolerance and in various analytical approaches. The nature of these applications often require galactosidases to be present in a

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