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G5635

Sigma-Aldrich

β-Galactosidase from Escherichia coli

Grade VIII, lyophilized powder, ≥500 units/mg protein

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Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Grade VIII

Quality Level

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

465 kDa

does not contain

BSA as extender

composition

Protein, ≥60% biuret

storage temp.

−20°C

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General description

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Application

β-Galactosidase is conjugated to an antibody which specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter. It may be used as a positive control protein with anti-β-galactosidase antibodies.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physical form

Contains Tris buffer salts and magnesium chloride

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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