G5893

Sigma-Aldrich

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

NACRES:
NA.72

Quality Level

form

fine powder

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

storage temp.

2-8°C

General description

Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.

Application

Gamborg′s B-5 basal medium with minimal organics has been used in culturing plant cells of Daucus carota. It has also been used for selection of primary transformants of Arabidopsis thaliana.
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
  • as a component of liquid media for the tissue culture of Brassica rapa
  • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
  • as a component of solid GM medium for germination of Arabidopsis seeds

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.

Pictograms

Flame over circle

Signal Word

Warning

Hazard Statements

RIDADR

UN 1486 5.1 / PGIII

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
Rebeca Gonzalez-Bayon et al.
Plant physiology, 180(1), 240-252 (2019-02-03)
Hybrids are used extensively in agriculture due to their superior performance in seed yield and plant growth, yet the molecular mechanisms underpinning hybrid performance are not well understood. Recent evidence has suggested that a decrease in basal defense response gene...
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
Leigh Mickelson-Young et al.
Journal of experimental botany, 67(21), 6077-6087 (2016-11-05)
The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on...
Jakob Petereit et al.
Frontiers in plant science, 8, 72-72 (2017-02-24)
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown...
Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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