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G6171

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal

clone G-A-5, purified from hybridoma cell culture

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Synonym(s):
GFAP Antibody - Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal, Gfap Antibody Sigma, Anti-GFAP
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

G-A-5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human, pig

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum.
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)
mouse ... Gfap(14580)
pig ... GFAP(396562)
rat ... Gfap(24387)

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This Item
SAB5201104MAB360MAB3402
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

G-A-5, monoclonal

clone

S206A-8, monoclonal

clone

GA5, monoclonal

clone

GA5, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

-

mol wt

antigen ~50 kDa

mol wt

antigen predicted mol wt 50 kDa

mol wt

-

mol wt

-

General description

Glial fibrillary acidic protein (GFAP) is intermediary filament present in astrocyte cells and is expressed in 10 different isoforms. It comprises of N-terminal head, central rod and C-terminal tail domains. GFAP is mapped to human chromosome 17q21.31.

Immunogen

purified GFAP from pig spinal cord.

Application

Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal has been used in immunocytochemistry and in immunofluorescence.
Monoclonal Anti-Glial Fibrillary Acidic Protein antibody is suitable for use in western blot and indirect immunofluorescence 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum). The antibody can also be used for immunoblot (approx. 50 kDa), immunohistochemistry (in paraformaldehyde-fixed, frozen rabbit brain sections) and immunocytochemistry.

Biochem/physiol Actions

Glial fibrillary acidic protein or GFAP assembly has been implicated in mitotic remodeling of glial structures. Genetic mutations in GFAP have been associated with Alexander disease.
Microdeletion in glial fibrillary acidic protein (GFAP) gene locus is implicated in intellectual disability and facial dysmorphism in children. High levels of GFAP is associated with acute intracerebral haemorrhage.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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TWO CASES WITH DIFFERENT EPILEPSY TYPE AND DYSMORPHIC FEATURES ASSOCIATED WITH 17q21. 31 MICRODELETION SYNDROME
Uctepe E, et al.
Genetic Counseling (Geneva, Switzerland), 27(3), 357-365 (2016)
Glial fibrillary acidic protein (GFAP) and the astrocyte intermediate filament system in diseases of the central nervous system
Hol EM and Pekny M
Current Opinion in Cell Biology, 32, 121-130 (2015)
Lipopolysaccharide differentially modulates expression of cytokines and cyclooxygenases in dorsal root ganglion cells via Toll-like receptor-4 dependent pathways
Tse KH, et al.
Neuroscience, 267, 241 -2251 (2014)
M Inagaki et al.
Brain pathology (Zurich, Switzerland), 4(3), 239-243 (1994-07-01)
Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein of astroglia, and belongs to the type III subclass of IF proteins. IF proteins are composed of an amino-terminal HEAD domain, a central ROD domain and a carboxyterminal TAIL
Yun Chen et al.
Journal of neuroimmunology, 334, 576978-576978 (2019-06-10)
Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by progressive learning and cognitive damage. Several hypotheses such as amyloid cascade hypothesis, hyper-phosphorylated τ hypothesis, and energy metabolism hypothesis have been proposed to elucidate the disease. However, the exact mechanism

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