G6171

Anti-Glial Fibrillary Acidic Protein Antibody

mouse monoclonal, G-A-5

• Synonym(s): Anti-GFAP
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00145904
• Conjugate: unconjugated
• Clone: G-A-5, monoclonal
• Application: ICC, IF, IHC, WB
• Citations: 103

Properties

• Product Name: Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal, clone G-A-5, purified from hybridoma cell culture
• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture, purified immunoglobulin
• antibody product type: primary antibodies
• clone: G-A-5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: rat, human, pig
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum., western blot: suitable
• isotype: IgG1
• UniProt accession no.: P14136
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 200
• Gene Information: human ... GFAP(2670) ; mouse ... Gfap(14580) ; pig ... GFAP(396562) ; rat ... Gfap(24387)

Description

General description

Glial fibrillary acidic protein (GFAP) is intermediary filament present in astrocyte cells and is expressed in 10 different isoforms. It comprises of N-terminal head, central rod and C-terminal tail domains. GFAP is mapped to human chromosome 17q21.31.

Immunogen

purified GFAP from pig spinal cord.

Application

Monoclonal Anti-Glial Fibrillary Acidic Protein antibody is suitable for use in western blot and indirect immunofluorescence 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum). The antibody can also be used for immunoblot (approx. 50 kDa), immunohistochemistry (in paraformaldehyde-fixed, frozen rabbit brain sections) and immunocytochemistry.

Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal has been used in immunocytochemistry and in immunofluorescence.

Biochem/physiol Actions

Glial fibrillary acidic protein or GFAP assembly has been implicated in mitotic remodeling of glial structures. Genetic mutations in GFAP have been associated with Alexander disease.

Microdeletion in glial fibrillary acidic protein (GFAP) gene locus is implicated in intellectual disability and facial dysmorphism in children. High levels of GFAP is associated with acute intracerebral haemorrhage.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable