To reconstitute dissolve G6378, Glucose-6-phosphate Dehydrogenase, in water and a dilute buffer. Following reconstitution, it should be refrigerated at 4°C, with stock solutions remaining stable for up to 6 months at this temperature.
G6378
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonym(s):
G-6-P-DH, Zwischenferment
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100 UNITS
$71.20
250 UNITS
$130.00
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$232.00
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$458.00
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$783.00
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type
Type XV
Quality Level
form
lyophilized powder
specific activity
200-400 units/mg protein (modified Warburg-Christian)
mol wt
128 kDa
purified by
crystallization
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
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Related Categories
1 of 4
This Item | G4134 | G5885 | G8404 |
|---|---|---|---|
| specific activity 200-400 units/mg protein (modified Warburg-Christian) | specific activity 200-400 units/mg protein (modified Warburg-Christian) | specific activity >= 550 units/mg protein (biuret) | specific activity ≥550 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension |
| application(s) diagnostic assay manufacturing | application(s) agriculture | application(s) agriculture | application(s) - |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 128 kDa | mol wt 128 kDa | mol wt 128 kDa | mol wt 128 kDa |
General description
Application
Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.[3]
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Physical form
Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight
Preparation Note
Prepared from G7877
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
María J Rodríguez-López et al.
Insects, 11(9) (2020-09-13)
A combination of biological control and host plant resistance would be desirable for optimally controlling the greenhouse whitefly, Trialeurodes vaporariorum in tomato crops. Whitefly settlement preference, oviposition, and survivorship were evaluated on ABL 10-4 and 'Moneymaker', two nearly-isogenic tomato lines
Protocols
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Related Content
Instructions
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What are the recommended reconstitution and storage procedures for item G6378, Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).
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What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?
1 answer-
The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.
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What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.
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