G6539

Sigma-Aldrich

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse

clone GFP-20, ascites fluid

Synonym(s):
GFP Antibody - Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, Gfp Antibody Sigma, Gfp Monoclonal Antibody
MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GFP-20, monoclonal

mol wt

antigen 27 kDa

contains

15 mM sodium azide

packaging

antibody small pack of 25 μL

application(s)

dot blot: suitable
indirect ELISA: suitable
western blot: 1:2,000 using purified recombinant GFP preparation

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.
The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is specific for GFP and GFP-labeled biomolecules.

Immunogen

GFP tagged fusion protein.
GFP tagged fusion protein

Application

Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes GFP (27 kDa) using immunoblotting, dot blot, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation and enzyme linked immunosorbent assay (ELISA). The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is suitable for use in western blot and ChIP assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Green Fluorescent Protein: Properties, Applications and Protocols (2005)
Justin M O'Sullivan et al.
Nature genetics, 36(9), 1014-1018 (2004-08-18)
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Dieckmann R, et al.
Molecular Biology of the Cell, 21(9), 1505-1518 (2010)
Gene loops juxtapose promoters and terminators in yeast
O'Sullivan JM, et al.
Nature Genetics, 36(9), 1014-1014 (2004)
Yanfeng Dai et al.
The Journal of biological chemistry, 286(22), 19905-19916 (2011-04-05)
CAPRI is a member of the GAP1 family of GTPase-activating proteins (GAPs) for small G proteins. It is known to function as an amplitude sensor for intracellular Ca(2+) levels stimulated by extracellular signals and has a catalytic domain with dual...

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