Unfortunately, the source species of cold-water fish for this product is not available. No sequence information is available either.
G7041
Fish Gelatin
Gelatin from cold water fish skin
1 of 2 reviewers received a sample product or took part in a promotion
from cold water fish skin, powder, suitable for cell culture and generation of scaffolds.
Synonym(s):
Gelatine, Teleostean gelatin
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100 G
$32.80
500 G
$106.00
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100 G
$32.80
500 G
$106.00
About This Item
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Product Name
Gelatin from cold water fish skin, solid
biological source
fish skin
Quality Level
product line
BioReagent
form
solid
mol wt
60 kDa
packaging
pkg of 100 g
pkg of 500 g
technique(s)
cell culture | mammalian: suitable
surface coverage
100—200 μg/cm2
impurities
≤2% Residue on ignition (Ash)
≤20 ppm Heavy metals
solubility
acetic acid: soluble
glycerol: soluble
water: soluble
shipped in
ambient
storage temp.
room temp
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General description
Application
Gelatin from cold water fish skin has been used in the preparation of water phase.[3] It has also been used to maintain growth in static culture[4][5] and in flavopiridol treatment and transcript labeling.[6]
Used as a blocking agent in immunochemistry.
Biochem/physiol Actions
Gelatin from fish skin can be used in the preparation of various gels based on their gelling characteristics. It can also be used as an additive in surimi processing to enhance the functional and mechanical properties of gel.[7]
Storage Class
11 - Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Protocols
This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.
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Hi, could you please let me know the fish species or the Amino Acid sequence of the gelatin? Thanks!
1 answer-
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What is the melting point of this specific fish gelatin?
1 answer-
Dry gelatin, when heated to 100 °C in the presence of air, swells and becomes soft, disintegrating to a carbonaceous mass. Solidified solutions may be reliquified at 37°C. Solutions of gelatin will undergo hydrolysis when heated above 40 °C for extended periods. Please see the link below to review additional information available in the Product Datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdfHelpful?
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What fish species is this gelatin product derived from? Thanks.
1 answer-
This gelatin product is produced from multiple cold water fish species. These include, but are not limited to, cod, pollock, and haddock.
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Is product G7041 acid pretreated or alkali pretreated?
1 answer-
This product is extracted from acid-cured tissue.
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Is this in solid powder form? Also, is it suitable to be used to form a hydrogel film?
1 answer-
Yes, this product is supplied in solid powder form. Gelatin, in general, can be used to form a hydrogel film, but this product has not been tested for that application.
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What is the molecular weight of this Gelatin?
What is the molecular weight of this Gelatin?
1 answer-
The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
Bloom Number Average Molecular Mass (Da)
50−125 (Low Bloom) 20,000−25,000
175−225 (Medium Bloom) 40,000−50,000
225−325 (High Bloom) 50,000−100,000Please see the link below to review the general datasheet for Gelatin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdfHelpful?
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Could you provide me with a preparation protocol to use it as a blocking agent in ELISA for G7041 ? And at what concentration is G7041 100 g.?
1 answer-
This powdered gelatin product is not specifically tested for use as a blocking agent in Elisa. Typically, Fish Skin Gelatin is prepared in a buffer at a concentration of 1 - 5% for this application. Please see the link below to review an article that may be helpful.
https://pubmed.ncbi.nlm.nih.gov/3611792/Please see the link below to review the general Gelatin datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdfHelpful?
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I would like to know the primary amine and functionable carboxylic acid group contents of this product.
1 answer-
The primary amine and functionable carboxylic acid group contents of G7041 were not determined in-house.
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How to store the product.
1 answer-
As stated in the PROPERTIES section, this item is stored at room temperature. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdfHelpful?
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How long fish gelatin(g7041) is stable from the moment it dissolves?
1 answer-
Sterile solutions of gelatin are quite stable when stored refrigerated. The 40 - 50% Solution of this product, G7765, is not assigned an expiration date, but it is retested every 2 years if stock remains. Please see the links below to review the general datasheet for gelatin products:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdfPlease see the link below to review a sample Certificate of Analysis for the prepared Fish Gelatin solution:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G7765-1LSLCQ7056.pdfHelpful?
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