G7163

α-Galactosidase, positionally specific from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(s): 1,6-alpha-D-galactoside galactohydrolase, alpha-Galactosidase, melibiase
• CAS Number: 9025-35-8
• Enzyme Commission number: 3.2.1.22 (BRENDA, IUBMB)
• MDL number: MFCD00130486

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: buffered aqueous solution
• specific activity: ≥20 units/mg protein
• mol wt: 80 kDa
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Escherichia coli CFT073 ... melA(1037886)

Description

Biochem/physiol Actions

Cleaves α(1→3)- and α(1→6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with live cells.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl α-D-galactopyranoside per min at pH 6.5 at 25 °C.

Physical form

This product is a sterile-filtered aqueous buffered solution.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable