MilliporeSigma
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G7396

Sigma-Aldrich

β-Glucuronidase from Escherichia coli

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

Synonym(s):
β-D-Glucuronide glucuronosohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type IX-A

Quality Level

form

lyophilized powder

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

mol wt

290 kDa

composition

Protein, 30-70% biuret

shipped in

wet ice

storage temp.

−20°C

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This Item
G8295G7646G8420
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

specific activity

≥10,000,000 units/g protein (30 min assay)

specific activity

5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

specific activity

≥20,000 units/mg protein

mol wt

290 kDa

mol wt

~290 kDa

mol wt

290 kDa

mol wt

290 kDa

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Contains buffer salts and stabilizer.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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