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G7396

Sigma-Aldrich

β-Glucuronidase from Escherichia coli

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type IX-A

Quality Level

form

lyophilized powder

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

mol wt

290 kDa

composition

Protein, 30-70% biuret

shipped in

wet ice

storage temp.

−20°C

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Related Categories

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Contains buffer salts and stabilizer.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bojan Šarkanj et al.
Analytica chimica acta, 1019, 84-92 (2018-04-08)
There is a critical need to better understand the patterns, levels and combinatory effects of exposures we are facing through our diet and environment. Mycotoxin mixtures are of particular concern due to chronic low dose exposures caused by naturally contaminated
Maria Papageorgiou et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 35(10), 2032-2044 (2018-08-18)
Deoxynivalenol (DON) is a Fusarium toxin, to which humans are frequently exposed via diet. Although the elderly are speculated to be sensitive to the toxic effects of DON as a result of age-related conditions, disease and altered DON metabolism, there
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine
Health Laboratory Science, 50, 286-289 (2004)
Enzymes
Hans Ulrich Bergmeyer
Methods of Enzymatic Analysis, 460-461 (1974)
Ping Wang et al.
Journal of analytical toxicology, 30(8), 570-575 (2006-11-30)
Confirmation of opioids in urine samples of clinical patients requires liberation of opioids from their glucuronide conjugates. Both acid hydrolysis and enzyme hydrolysis using beta-glucuronidase from various sources have been reported, with the latter approach prevailing in most clinical toxicology

Articles

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Protocols

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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