This enzyme is lyophilized from a solution containing buffer salts and a stabilizer. The product may be reconsituted at 1 mg/mL in water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/412/974/g7646dat.pdf
G7646
β-Glucuronidase from Escherichia coli
Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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type
Type VII-A
Quality Level
form
lyophilized powder
specific activity
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
mol wt
290 kDa
composition
Protein, ~25% biuret
storage temp.
−20°C
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Related Categories
1 of 4
This Item | G7396 | G8420 | G0799 |
|---|---|---|---|
| specific activity 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | specific activity 1,000,000-5,000,000 units/g protein (30 min assay) | specific activity ≥20,000 units/mg protein | specific activity >20,000,000 units/g protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous glycerol solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 290 kDa | mol wt 290 kDa | mol wt 290 kDa | mol wt 74 kDa |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| type Type VII-A | type Type IX-A | type - | type - |
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Contains buffer salts and stabilizer.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M Coulet et al.
Journal of veterinary pharmacology and therapeutics, 25(2), 89-97 (2002-05-10)
The pharmacokinetics of ibafloxacin, a new veterinary fluoroquinolone antimicrobial agent, was studied following intravenous (i.v.) and oral administration to healthy dogs. The mean absolute bioavailability of ibafloxacin after oral doses of 7.5, 15 and 30 mg/kg ranged from 69 to
Lawrence D Joh et al.
Biotechnology progress, 22(3), 723-730 (2006-06-03)
Extraction and storage of a recombinant protein produced by transient expression following agroinfiltration of lettuce were investigated. Lettuce leaves expressing beta-glucuronidase (GUS) were extracted by homogenization in several buffer combinations, and the yield and stability were assessed. The reducing agent
Niina O Nurmi et al.
Primates; journal of primatology, 64(2), 215-225 (2022-12-25)
In mammals, the costs of reproduction are biased towards females. Lactation is particularly energetically expensive, and behavioral and physiological data indicate that maternal effort during lactation induces energetic stress. Another source of stress in females is male aggression directed towards
H Mita et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 34(8), 1262-1269 (2004-08-10)
Aspirin challenge of aspirin-intolerant asthma (AIA) patients causes a significant increase in leukotriene E4 (LTE4) concentration in urine. However, knowledge on leukotriene B4 (LTB4) generation in patients with AIA is insufficient. Recent research has demonstrated that exogenously administered LTB4 is
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 40, 286-289 (2004)
Articles
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Protocols
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
Related Content
Datasheet
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It is recommended to resuspend the product in which buffer?
1 answer-
Helpful?
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Is BSA or other protein components included in the overall percentage listed on the CoA for the product G7646, which is β-Glucuronidase from Escherichia coli (CAS 9001-45-0)?
1 answer-
No. BSA or other protein sources are added to the product except Potassium Phosphate and Sucrose. Although this is a fermentation product, there may be other protein residues in it in a negligible amount. The product is tested by activity, residual protein contaminants are not tested. The protein percentage is the total protein (tested by Biuret method).
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