G7654
Gel Loading Solution
for NA electrophoresis, solution
Synonym(s):
DNA Gel Loading Solution, Gel Loading Buffer
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mL | Estimated to ship TODAYfromMILWAUKEE | $33.10 |
About This Item
NACRES:
NA.25
UNSPSC Code:
12161703
$33.10
Estimated to ship TODAYDetails
General description
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
Preparation Note
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
Other Notes
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
1 of 1
This Item | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| concentration 6 × | concentration - | concentration - | concentration 1.25 × |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form solution | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. −20°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
David R Macinga et al.
Antimicrobial agents and chemotherapy, 47(8), 2526-2537 (2003-07-25)
We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
S Henry et al.
Vox sanguinis, 70(1), 21-25 (1996-01-01)
While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G7654-5ML | 04061838099358 |