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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

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Synonym(s):
blocking buffer
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

powder blend

storage temp.

room temp

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This Item
B6429W0138T7527
Sigma-Aldrich

Sigma-Aldrich

G7663

Gelatin blocking buffer

-
Tris-Borate-EDTA buffer BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
form

powder blend

form

solution

form

-

form

powder blend

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service