G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

NACRES:
NA.25

Quality Level

grade

for molecular biology

form

powder blend

storage temp.

room temp

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Packaging

1 L in poly bottle

Application

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Daisuke Tomida et al.
Investigative ophthalmology & visual science, 52(6), 3193-3199 (2011-02-08)
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Iram Liaqat et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 43(3), 969-980 (2012-07-01)
Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim...
A sensitive cell-based assay for the detection of residual infectious West Nile virus.
Koldijk, M.H., et al.
Vaccine, 25, 39-39 (2007)
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand...
K A Brogden et al.
Infection and immunity, 67(8), 4256-4259 (1999-07-23)
Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In...
Protocols
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
Read More
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Read More

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