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G8132

Sigma-Aldrich

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
EC Number:
3.2.1.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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biological source

limpet (Patella vulgata)

Quality Level

300

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

Storage temp.

−20°C

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1 of 4

This Item
G1512G0376G7396
β-Glucuronidase from limpets (Patella vulgata) Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Sigma-Aldrich

G8132

β-Glucuronidase from limpets (Patella vulgata)

β-Glucuronidase from Helix pomatia Type H-5, lyophilized powder, ≥400,000 units/g solid

Sigma-Aldrich

G1512

β-Glucuronidase from Helix pomatia

β-Glucuronidase from bovine liver Type B-3, ≥2,000,000 units/g solid

Sigma-Aldrich

G0376

β-Glucuronidase from bovine liver

β-Glucuronidase from Escherichia coli Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

Sigma-Aldrich

G7396

β-Glucuronidase from Escherichia coli

biological source

limpet (Patella vulgata)

biological source

-

biological source

bovine liver

biological source

-

specific activity

1,000,000-3,000,000 units/g solid

specific activity

≥400,000 units/g solid

specific activity

≥2,000,000 units/g solid

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

form

lyophilized powder

form

lyophilized powder

form

solid

form

lyophilized powder

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

Application

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine. [1]
New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates.[2] In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate.

Analysis Note

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000489744
0000489744
0000457770
0000457770
0000457769

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Robert West et al.
Pain physician, 13(1), 71-78 (2010-02-02)
Physicians determine patient compliance with their medications by use of urine drug testing. It is known that measurement of benzodiazepines is limited by immunoassay specificity and cutoff limits and therefore does not offer physicians an accurate picture of their patients'
Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>
S.V. Marathe &amp; J.E. McEwen
Gene, 154, 105-107 (1994)
J D McCarter et al.
Current opinion in structural biology, 4(6), 885-892 (1994-12-01)
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Catalytic mechanisms of enymatic glycosyl transfer
M.L. Sinnott
Chemical Reviews, 90, 1171-1202 (1990)
View All Related Papers

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Analysis of Drugs of Abuse in Urine

Validate urine sample cleanup with HLB SPE for opioid analysis via LC-MS/MS using the Supel™ Swift HLB 96-well plate.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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