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G8168

Sigma-Aldrich

G 418 disulfate salt solution

50 mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture

Synonym(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
692.71
MDL number:
PubChem Substance ID:
NACRES:
NA.76

biological source

Micromonospora echinospora

Quality Level

sterility

0.1 μm filtered

product line

BioReagent

form

solution

potency

50-60 mg per mL

mol wt

692.7

packaging

pkg of 10 and 100 mL

concentration

50 mg/mL in H2O

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

colorless

solubility

H2O: 50 mg/mL, clear

antibiotic activity spectrum

parasites

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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General description

Chemical structure: aminoglycoside

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Caution

The product is stable for two years when stored at 2-8°C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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