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G8420

β-Glucuronidase from Escherichia coli

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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25000 UNITS

$174.00

100000 UNITS

$570.00

$174.00

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About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
MFCD00199013
EC Number:
3.2.1.31 (BRENDA, IUBMB)
Specific activity:
≥20,000 units/mg protein
Recombinant:
expressed in E. coli overproducing strain

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recombinant

expressed in E. coli overproducing strain

form

lyophilized powder

specific activity

≥20,000 units/mg protein

mol wt

290 kDa

composition

, ~25%

storage temp.

−20°C

Quality Level

300

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1 of 4

This Item
G8295G7396G0751
β-Glucuronidase from Escherichia coli ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8420

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8295

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

Sigma-Aldrich

G7396

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Helix pomatia Type H-1, partially purified powder, ≥300,000 units/g solid

Sigma-Aldrich

G0751

β-Glucuronidase from Helix pomatia

specific activity

≥20,000 units/mg protein

specific activity

≥10,000,000 units/g protein (30 min assay)

specific activity

1,000,000-5,000,000 units/g protein (30 min assay)

specific activity

≥300,000 units/g solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

partially purified powder

recombinant

expressed in E. coli overproducing strain

recombinant

expressed in E. coli overproducing strain

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

290 kDa

mol wt

~290 kDa

mol wt

290 kDa

mol wt

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000436125
0000436125
0000252708
0000250210
0000235771

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Find documentation for the products that you have recently purchased in the Document Library.

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Enzymes
Hans Ulrich Bergmeyer
Methods of Analysis, 2, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
Fei Song et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 67(11-12), 611-619 (2013-02-19)
Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system
View All Related Papers

Articles

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Related Content

Data Sheet - G8420

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