G8507

Sigma-Aldrich

α-Galactosidase from green coffee beans

ammonium sulfate suspension, ≥9 units/mg protein

Synonym(s):
alpha-Galactosidase, Melibiase, α-D-Galactoside galactohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

Quality Level

form

ammonium sulfate suspension

specific activity

≥9 units/mg protein

storage temp.

2-8°C

General description

α-Galactosidase from green coffee beans is encoded by the GLA gene.

Application

α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

Packaging

25 units in glass bottle

Biochem/physiol Actions

α-Galactosidase from green coffee beans cleaves the terminal α-galactose from the oligosaccharides. α-Galactosidase deficiency is associated with Fabry disease.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per min at pH 6.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0, containing BSA

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Pig xenogeneic antigen modification with green coffee bean alpha-galactosidase
Luo Y, et al.
Xenotransplantation, 6(4), 238-248 (1999)
A prophylactic alpha-Gal-based glycovaccine effectively protects against murine acute Chagas disease
Portillo S, etal.
NPJ vaccines, 4(1), 13-13 (2019)
Amblyomma sculptum tick saliva: alpha-Gal identification, antibody response and possible association with red meat allergy in Brazil
Araujo RN, et al.
International Journal For Parasitology, 46(3), 213-220 (2016)
Structure-function relationships in alpha-galactosidase A
Garman SC
Acta Paediatrica (Oslo, Norway : 1992), 96, 6-16 (2007)
Orthoester functionalized N-guanidino derivatives of 1, 5-dideoxy-1, 5-imino-d-xylitol as pH-responsive inhibitors of beta-glucocerebrosidase
Sevvsek A, et al.
MedChemComm, 8(11), 2050-2054 (2017)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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