G8507

α-Galactosidase from green coffee beans

ammonium sulfate suspension, ≥9 units/mg protein

• Synonym(s): alpha-Galactosidase, α-D-Galactoside galactohydrolase, Melibiase
• CAS Number: 9025-35-8
• Enzyme Commission number: 3.2.1.22 (BRENDA, IUBMB)
• MDL number: MFCD00130486
• NACRES: NA.54

Properties

• form: ammonium sulfate suspension
• Quality Level: 200
• specific activity: ≥9 units/mg protein
• storage temp.: 2-8°C

Description

General description

α-Galactosidase from green coffee beans is encoded by the GLA gene.

Application

α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

Packaging

1 unit

5, 25, 50 units

Biochem/physiol Actions

α-Galactosidase from green coffee beans cleaves the terminal α-galactose from the oligosaccharides. α-Galactosidase deficiency is associated with Fabry disease.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per min at pH 6.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6.0, containing BSA

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable