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G8507

Sigma-Aldrich

α-Galactosidase from green coffee beans

ammonium sulfate suspension, ≥9 units/mg protein

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Synonym(s):
alpha-Galactosidase, α-D-Galactoside galactohydrolase, Melibiase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

specific activity

≥9 units/mg protein

storage temp.

2-8°C

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β-Galactosidase from bovine testes

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

lyophilized powder

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

α-Galactosidase from green coffee beans is encoded by the GLA gene.

Application

α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

Biochem/physiol Actions

α-Galactosidase from green coffee beans cleaves the terminal α-galactose from the oligosaccharides. α-Galactosidase deficiency is associated with Fabry disease.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per min at pH 6.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0, containing BSA

inhibitor

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Eva Iniguez et al.
PLoS neglected tropical diseases, 11(10), e0006039-e0006039 (2017-10-27)
Protozoan parasites from the genus Leishmania cause broad clinical manifestations known as leishmaniases, which affect millions of people worldwide. Cutaneous leishmaniasis (CL), caused by L. major, is one the most common forms of the disease in the Old World. There
A prophylactic alpha-Gal-based glycovaccine effectively protects against murine acute Chagas disease
Portillo S, etal.
NPJ vaccines, 4(1), 13-13 (2019)
Amblyomma sculptum tick saliva: alpha-Gal identification, antibody response and possible association with red meat allergy in Brazil
Araujo RN, et al.
International Journal For Parasitology, 46(3), 213-220 (2016)
Pig xenogeneic antigen modification with green coffee bean alpha-galactosidase
Luo Y, et al.
Xenotransplantation, 6(4), 238-248 (1999)
Structure-function relationships in alpha-galactosidase A
Garman SC
Acta Paediatrica (Oslo, Norway : 1992), 96, 6-16 (2007)

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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