G8877

Guanosine 5′-triphosphate sodium salt hydrate

≥95% (HPLC), RNA synthesis substrate, powder

• Synonym(s): GTP
• Empirical Formula (Hill Notation): C₁₀H₁₆N₅O₁₄P₃ · xNa⁺
• CAS Number: 36051-31-7
• Molecular Weight: 523.18 (free acid basis)
• NACRES: NA.77
• PubChem Substance ID: 24895354
• UNSPSC Code: 41106305
• EC Number: 252-847-2
• MDL number: MFCD00077781
• Beilstein/REAXYS Number: 3585677
• Assay: ≥95% (HPLC)
• Form: powder
• Quality level: 200

Properties

• Product Name: Guanosine 5′-triphosphate sodium salt hydrate, ≥95% (HPLC), powder
• InChI key: KZRMTEVIDYXWQW-CYCLDIHTSA-K
• InChI: 1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• assay: ≥95% (HPLC)
• form: powder
• color: white
• solubility: H₂O: 50 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... HRAS(3265)

Description

Application

Guanosine 5′-triphosphate sodium salt hydrate has been used:

• in the preparation of horseradish peroxidase (HRP)-modified platination reagents[1]
• in whole cell, voltage-clamp recordings
• in in vitro inhibition of transglutaminase (tTG)

Biochem/physiol Actions

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. GTP activates the signal transducing G proteins which are involved in various cellular processes including proliferation, differentiation, and activation of several intracellular kinase cascades. Proliferation and apoptosis are regulated in part by the hydrolysis of GTP by small GTPases Ras and Rho. Another type of small GTPase, Rab, plays a role in the docking and fusion of vesicles and may also be involved in vesicle formation. In addition to its role in signal transduction, GTP also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA.

Guanosine-5′-triphosphate is a purine nucleotide that acts as a substrate for the synthesis of RNA during the transcription. GTP is a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. Activates the signal transducing G proteins involved in proliferation, differentiation, and activation of several intracellular kinase cascades.

Features and Benefits

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Preparation Note

Prepared by enzymatic phosphorylation of 5′-GMP.

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type N95 (US)