G8885

β-Glucuronidase from Helix pomatia

Type H-3, aqueous solution, ≥90,000 units/mL

• Synonym(s): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• EC Number: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type H-3
• Quality Level: 200
• form: aqueous solution
• specific activity: ≥90,000 units/mL
• secondary activity: ≤1,000 units/mL sulfatase
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:

• to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection[1]
• as a component in the protoplasting solution[2]
• for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)[3]
• to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples[4]

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo. [5]

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable