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G9277

Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

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Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

biological source

synthetic (organic)

Quality Level

grade

for molecular biology

Assay

≥99%

form

powder or crystals

storage condition

(Tightly closed. Dry. )

technique(s)

RNA extraction: suitable

color

colorless to white,

mp

120-122 °C (lit.)

solubility

water: 100 mg/mL, clear, colorless

density

1.29 g/cm3 at 20 °C (68 °F)

cation traces

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

application(s)

agriculture

foreign activity

DNase, RNase, none detected

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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1 of 4

This Item
G66395098350980
Guanidine thiocyanate for molecular biology, ≥99%

Sigma-Aldrich

G9277

Guanidine thiocyanate

-
Guanidine thiocyanate ≥97% (titration)

Sigma-Aldrich

G6639

Guanidine thiocyanate

-
Guanidine thiocyanate solution BioUltra, for molecular biology, ~6 M in H2O

Sigma-Aldrich

50983

Guanidine thiocyanate solution

Premium Grade
Guanidine thiocyanate BioUltra, for molecular biology, ≥99.0% (AT)

Sigma-Aldrich

50980

Guanidine thiocyanate

Premium Grade
assay

≥99%

assay

≥97% (titration)

assay

-

assay

≥99.0% (AT)

form

powder or crystals

form

powder or crystals

form

-

form

solid

storage condition

(Tightly closed. Dry. )

storage condition

-

storage condition

-

storage condition

-

technique(s)

RNA extraction: suitable

technique(s)

RNA extraction: suitable

technique(s)

-

technique(s)

-

color

colorless to white,

color

white to off-white

color

-

color

-

General description

Guanidine thiocyanate, or guanidinium rhodanide, is a versatile chemical compound widely used in molecular biology and biochemical research. Acting as a chaotropic agent, it disrupts cell and organelle membranes, facilitating cell lysis and organelle isolation. Notably, it serves as a potent protein denaturant, surpassing guanidine hydrochloride in unfolding proteins. This property is valuable for purifying nucleic acids from crude cell lysates, effectively separating nucleic acids from denatured proteins.

In nucleic acid purification, guanidine thiocyanate is effective in inactivating DNases and RNases, preventing nucleic acid degradation and ensuring favorable yields. Its dual denaturing and enzyme-inhibiting properties make it crucial for DNA and RNA studies. Beyond nucleic acid isolation, guanidine thiocyanate is used for cell lysis and virus particle inactivation during RNA and DNA extractions. It not only disrupts membranes but also denatures RNase and DNase enzymes, protecting extracted nucleic acids from enzymatic degradation. The versatility of guanidine thiocyanate extends to molecular biology techniques like PCR, Northern blotting, and Southern blotting. It serves as a vital reagent in these methods, contributing to the successful analysis and amplification of nucleic acids.

Application

Guanidine thiocyanate has been used:
  • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
  • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
  • for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Features and Benefits

  • Highly versatile surfactant for your cell biology and biochemical research
  • Suitable for RNA extraction in sensitive molecular biology applications
  • Free from Dnase and Rnase
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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