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G9282

Sigma-Aldrich

Monoclonal Anti-Glutamate antibody produced in mouse

clone GLU-4, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GLU-4, monoclonal

contains

15 mM sodium azide

species reactivity

wide range

application(s)

indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating

isotype

IgG1

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Specificity

The antibody reacts specifically with L-glutamate (L-glutamic acid), when immobilized with glutaraldehyde. Cross-reaction is observed with Gly-Glu and Asp-Glu. When using the product at the recommended working dilution, in indirect and competitive ELISA, weak cross-reaction may be obtained with D-glutamate, L-glutamine, L-aspartate, D-aspartate, L-asparagine, β-alanine, glycine, 5-aminovaleric acid, γ-aminobutyric acid (GABA) and Gly-Asp. Glutamate is found in cell bodies, axons, and terminals of glutamatergic neurons in the CNS.

Immunogen

L-glutamic acid (Glu).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:
  • immunolabeling of glutamate in the lamina of the Drosophila visual system
  • immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria
  • immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice
  • use as a primary antibody to measure the glutamate release in synaptosome preparations
It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating.

Biochem/physiol Actions

Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington′s disease, Alzheimer′s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Hiroshi Ando et al.
The Journal of experimental biology, 207(Pt 26), 4663-4677 (2004-12-08)
The heart of Squilla oratoria contains a cardiac ganglion that consists of 15 intrinsic neurons, supplied by a pair of inhibitory nerves and two pairs of excitatory nerves, arising from the central nervous system. These comprise the extrinsic cardiac innervation....
Petrusz P, et al.
Techniques in Immunocytochemistry, 4, 253-253 (1989)
Ling-Qiang Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(45), 12211-12220 (2007-11-09)
Activation of glycogen synthase kinase-3 (GSK-3) can cause memory deficits as seen in Alzheimer's disease, the most common age-associated dementia, but the mechanism is not understood. Here, we found that activation of GSK-3 by wortmannin or transient overexpression of wild-type...
A lot of "excitement' about neurodegeneration.
R Taylor
Science (New York, N.Y.), 252(5011), 1380-1381 (1991-06-07)
S Seo et al.
Neuroscience, 192, 28-36 (2011-07-23)
Agmatine, the decarboxylated metabolite of l-arginine, is considered to be a novel putative neurotransmitter. Recent studies have demonstrated that endogenous agmatine may directly participate in the processes of spatial learning and memory. Agmatine-immunoreactivity has been observed within synaptic terminals of...

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