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G9282

Sigma-Aldrich

Monoclonal Anti-Glutamate antibody produced in mouse

clone GLU-4, ascites fluid

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MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GLU-4, monoclonal

contains

15 mM sodium azide

species reactivity

wide range

technique(s)

indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GRM1(2911)

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

GLU-4, monoclonal

clone

AGP-47, monoclonal

clone

GT-34, monoclonal

clone

1F7, monoclonal

species reactivity

wide range

species reactivity

human, baboon (weakly)

species reactivity

sheep, goat, bovine

species reactivity

human

technique(s)

indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating

technique(s)

dot blot: suitable, indirect ELISA: suitable, western blot: 1:10,000

technique(s)

indirect ELISA: 1:10,000-1:20,000

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

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Specificity

The antibody reacts specifically with L-glutamate (L-glutamic acid), when immobilized with glutaraldehyde. Cross-reaction is observed with Gly-Glu and Asp-Glu. When using the product at the recommended working dilution, in indirect and competitive ELISA, weak cross-reaction may be obtained with D-glutamate, L-glutamine, L-aspartate, D-aspartate, L-asparagine, β-alanine, glycine, 5-aminovaleric acid, γ-aminobutyric acid (GABA) and Gly-Asp. Glutamate is found in cell bodies, axons, and terminals of glutamatergic neurons in the CNS.

Immunogen

L-glutamic acid (Glu).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:
  • immunolabeling of glutamate in the lamina of the Drosophila visual system
  • immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria
  • immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice
  • use as a primary antibody to measure the glutamate release in synaptosome preparations
It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating.

Biochem/physiol Actions

Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington′s disease, Alzheimer′s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Hiroshi Ando et al.
The Journal of experimental biology, 207(Pt 26), 4663-4677 (2004-12-08)
The heart of Squilla oratoria contains a cardiac ganglion that consists of 15 intrinsic neurons, supplied by a pair of inhibitory nerves and two pairs of excitatory nerves, arising from the central nervous system. These comprise the extrinsic cardiac innervation.
Ling-Qiang Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(45), 12211-12220 (2007-11-09)
Activation of glycogen synthase kinase-3 (GSK-3) can cause memory deficits as seen in Alzheimer's disease, the most common age-associated dementia, but the mechanism is not understood. Here, we found that activation of GSK-3 by wortmannin or transient overexpression of wild-type
A lot of "excitement' about neurodegeneration.
R Taylor
Science (New York, N.Y.), 252(5011), 1380-1381 (1991-06-07)
Yun Zhang et al.
The journal of headache and pain, 21(1), 72-72 (2020-06-12)
Vestibular migraine has recently been recognized as a novel subtype of migraine. However, the mechanism that relate vestibular symptoms to migraine had not been well elucidated. Thus, the present study investigated vestibular dysfunction in a rat model of chronic migraine
Heika Hildebrandt et al.
PloS one, 6(8), e23686-e23686 (2011-09-03)
Ablation of a cochlea causes total sensory deafferentation of the cochlear nucleus in the brainstem, providing a model to investigate nervous degeneration and formation of new synaptic contacts in the adult brain. In a quantitative electron microscopical study on the

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