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G9422

β-Glycerophosphate disodium salt hydrate

BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

Synonym(s):

BGP, Glycerol 2-phosphate disodium salt hydrate

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Size/SKUAvailabilityPrice
10 g

Estimated to ship onAugust 03, 2026fromMILWAUKEE

$86.50
50 g

Estimated to ship onAugust 03, 2026fromMILWAUKEE

$228.00
100 g

Estimated to ship onAugust 03, 2026fromMILWAUKEE

$382.00
500 g

Estimated to ship onAugust 03, 2026fromMILWAUKEE

$1,340.00

About This Item

Linear Formula:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS Number:
Molecular Weight:
216.04 (anhydrous basis)
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352201
EC Number:
212-464-3
MDL number:

$86.50


Estimated to ship onAugust 03, 2026Details


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biological source

synthetic (chemical)

Quality Segment

product line

BioUltra

assay

≥99% (titration)

form

powder

technique(s)

cell culture | mammalian: suitable, cell culture | plant: suitable

impurities

≤0.1% Insoluble matter, ≤0.5 mol % L-α-isomer

color

white

mp

102-104 °C (lit.)

solubility

water: soluble

anion traces

chloride (Cl-): ≤0.05%, sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.001%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.001%, K: ≤0.005%, Mg: ≤0.0005%, Pb: ≤0.001%, Zn: ≤0.0005%

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

General description

Glycerophosphoric acid disodium salt hydrate, also known as BGP, is a versatile compound with applications across various research domains, including cell biology, biochemical research, and metabolomics. Recognized as an endogenous metabolite and a serine-threonine phosphatase inhibitor, BGP is extensively utilized in chitosan hydrogel formulations for drug delivery research. It serves as a phosphate source for cell growth and recombinant protein production, offering advantages in media formulations by reducing precipitation at higher pH levels. Additionally, BGP finds applications in micro-arc oxidation electrolyte solutions, contributing to the creation of photocatalytic coatings.
In cell biology, β-Glycerophosphate disodium salt plays a pivotal role as a phosphate group donor in matrix mineralization studies and accelerates calcification in vascular smooth muscle cells. It also promotes bone matrix mineralization when delivered to osteoblasts, providing a crucial source of phosphate ions. In the realm of biochemical research, BGP functions as a classical serine-threonine phosphatase inhibitor in kinase reaction buffers, offering broad-spectrum inhibition. Its role as an organic phosphate donor is particularly valuable in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. Moreover, BGP is utilized to buffer M17 media for Lactococcus culture in recombinant protein expression. The comprehensive utility of BGP spans multiple areas of research, making it an essential component in studies encompassing cell biology, biochemical research, and metabolomics.

Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Cell culture and Plant cell culture
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • BioUltra Grade for your Cell Biology and Biochemical research

Analysis Note

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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This Item
G542261668S5136
technique(s)

cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

biological source

synthetic (chemical)

biological source

synthetic

biological source

-

biological source

-

assay

≥99% (titration)

assay

-

assay

≥95.0% (TLC), 95-102% anhydrous basis (corrected for sodium, T)

assay

≥99.0%

solubility

water: soluble

solubility

water: 50 mg/mL, clear, colorless

solubility

-

solubility

water: soluble 100 mg/mL

form

powder

form

powder

form

powder or solid

form

powder or crystals

impurities

≤0.1% Insoluble matter, ≤0.5 mol % L-α-isomer

impurities

≤0.1% Inorganic phosphorus, ≤1.0 mol % L-α-isomer

impurities

≤35% water

impurities

-


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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