G9785

GSEM Supplement

liquid, suitable for cell culture,

• Synonym(s): Glutamine synthetase expression medium supplement
• MDL number: MFCD00677096
• UNSPSC Code: 12352205
• NACRES: NA.75

Properties

• Product Name: GSEM Supplement, 50 ×, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• Quality Level: 200
• form: liquid
• concentration: 50 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium.[1] It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.[2]

Application

GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells[3] and selective medium for NS0 myeloma cells.[4]

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H₂O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable