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G9880

Sigma-Aldrich

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

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Synonym(s):
L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
CAS Number:
Enzyme Commission number:
Beilstein/REAXYS Number:
3562140
EC Number:
MDL number:
NACRES:
NA.54

biological source

Porcine heart

Quality Level

form

ammonium sulfate suspension

specific activity

≥75 units/mg protein

mol wt

42 kDa

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.05%

shipped in

wet ice

storage temp.

2-8°C

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G8255G2751C3260
vibrant-m

C3260

Citrate Synthase from porcine heart

specific activity

≥75 units/mg protein

specific activity

≥75 units/mg protein

specific activity

200-500 units/mg protein

specific activity

≥100 units/mg protein

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.05%

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%, glutamic-oxalacetic transaminase ≤0.1%

foreign activity

glutamic-pyruvic transaminase ≤0.03%, lactic dehydrogenase ≤0.01%, malic dehydrogenase ≤0.01%

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%

mol wt

42 kDa

mol wt

-

mol wt

-

mol wt

98 kDa ( 49 kDa monomer)

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Suspension in 1.8 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Martin J J Ronis et al.
Experimental biology and medicine (Maywood, N.J.), 238(2), 151-162 (2013-04-12)
Metabolic syndrome is often accompanied by development of hepatic steatosis and less frequently by non-alcoholic fatty liver disease (NAFLD) leading to non-alcoholic steatohepatitis (NASH). Replacement of corn oil with medium chain triacylglycerols (MCT) in the diets of alcohol-fed rats has
Xing-Fei Pan et al.
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, 26(6), 477-479 (2013-05-01)
To investigate the level of the serum IL-21 and its correlation with serum biochemical indices of liver function test in patients with acute-on-chronic liver failure. Sixty patients with acute-on-chronic liver failure (severe hepatitis group) and 18 normal cases (control group)
Jennifer Zechel et al.
Methods in molecular biology (Clifton, N.J.), 399, 79-98 (2008-03-04)
Mitochondrial dysfunction has been increasingly shown as a critical process that makes certain areas of the brain more susceptible not only to neurological disease but also to aging. Quantitative histochemistry is a series of procedures for measuring select metabolites in
Hua Du
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control, 25(1), 115-115 (2013-05-22)
To discuss the relationship between advanced schistosomiasis and HBV infection. A total of 250 advanced schistosomiasis patients were examined with ultrasound, and their serum samples were detected for liver function, HBsAg, etc. The correlations between HBV infection and advanced types
Antiviral immune response after live yellow fever vaccination of a kidney transplant recipient treated with IVIG.
Mark K Slifka et al.
Transplantation, 95(9), e59-e61 (2013-05-08)

Articles

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