If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
GAHK20
Glucose (HK) Assay Kit
sufficient for 20 assays
Synonym(s):
Glucose Quantification Kit
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Check Cart for Availability | $89.00 |
About This Item
usage
sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (glucose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
General description
Quantitative measurement of glucose in serum, plasma, urineor culture media underpins metabolic research (including flux analysis andinsulin-signaling studies), and disease modeling (such as the Warburg effect incancer and investigations into oxidative-stress pathways or glycogen storagedisorders).
The GAHK20 enzymatic glucose assay kit, based on theglucose/hexokinase reaction offers high sensitivity, a broad linear range, andcompatibility with high-throughput screening and detailed mechanistic studiesof metabolic health and disease.
1 of 1
This Item | |||
|---|---|---|---|
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| usage sufficient for 20 assays | usage sufficient for 20 assays | usage sufficient for 20 assays | usage sufficient for 20 assays |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| analyte chemical class(es) sugars (glucose) | analyte chemical class(es) - | analyte chemical class(es) sugars (fructose) | analyte chemical class(es) sugars |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| technique(s) photometry: suitable | technique(s) activity assay: suitable | technique(s) photometry: suitable | technique(s) photometry: suitable |
Kit Components Only
- glucose (HK) assay reagent 20 mL
- glucose standard .5 mL
Storage Class
10 - Combustible liquids
wgk
WGK 1
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GAHK20-1KT | 04061833651414 |
| G3293-50ML | 04061837904998 |
| G3293-20ML | 04061837904981 |
| G3285-.5ML | 04061833629833 |
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How can I determine the shelf life / expiration / retest date of this product?
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I tested a glucose reference solution at a concentration of 1 mg/ml using a sample volume of 0.1 ml and 1 ml of reagent. The measured extinction was 2.33, which is too high. After diluting the solution to a concentration of 0.1 mg/ml, the value was 0,36.
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You have done the correct thing in diluting the glucose reference solution 10-fold; the DF (dilution factor) would therefore be 10. The calculation for the diluted sample is (0.36) X (1.1) X (10) X (0.029)/0.1, which equals 1.1 mg glucose per mL for the 1 mg/mL glucose reference solution.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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The procedure says that solutions that are strongly colored should be decolorized for the kit to work. Do you have any suggestions on how to decolorize my sample?
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Solutions may be decolorized by adding a small amount of charcoal, stirring a few moments, and then clarifying by filtration or centrifugation to remove the charcoal. An activated charcoal product, such as item 161551 would be appropriate. See the link below to review this product:
https://www.sigmaaldrich.com/product/sigald/161551Helpful?
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I want to detect the change of glucose concentration in macrophages. How to prepare the sample
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A macrophage sample should be lysed using a cell lysis protocol similar to one used for Western Blotting. A useful lysis protocol can be found linked below.
Following cell lysis, the sample should be diluted with deionized water to 0.05 to 5 mg of glucose per mL. The sample should be deproteinized (using a common perchloric acid precipitation procedure) and filtered to clarify the solution. Any solutions that are strongly colored should be decolorized using a decolorization agent such as activated charcoal. Any samples in which gases are liberated during the sample preparation process should be degassed prior to analysis.
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How to adapt the Glucose Assay Kit to a 96-well plate?
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It may be possible to adapt this kit for use in a 96-well plate format. This would require method development and reagent scale down by the end user. There are no specific instructions available. Should a 96-well assay be preferred or required, please see product MAK476 at the link below. This product has been optimized for use in this format.
https://www.sigmaaldrich.com/product/sigma/mak476Helpful?
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Is the product G3293-20ML included in the GAHK20-1KT kit?
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Yes, the item G3293-20ML is a component of the complete GAHK20-1KT kit. Therefore, if G3293-20ML is on backorder, it can be obtained by ordering the GAHK20-1KT.
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Is GAHK20 assay compatible with C. elegans worm lysates? What buffer you recommed to prepare lysates?
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The Glucose (HK) Assay Kit has been optimized for use in food and other crude materials. This kit has not been qualified for use with cells or organisms such as C. elegans. The researcher would have to determine suitability, however there is some indication that other customers have been successful. See the link below for a publication that may be of interest:
https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1005561&type=printableNote that samples may require centrifugation, filtration or deproteinization to further clarify.
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How do I degas a solution for use with Product GAHK20, Glucose (HK) Assay Kit?
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You can degas a solution by either putting it under a vacuum or by bubbling nitrogen through it.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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