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Sepharose® 6B

Cytiva 17-0110-01, pack of 1 L

Agarose, Beaded
CAS Number:
MDL number:

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


autoclavable Autoclavable, 20 min at 120°C in pH 7


pack of 1 L


6% agarose

particle size

45-165 μm

cleaning in place

4 - 9

working range

4 - 9

SMILES string




InChI key


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General description

Sepharose® 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose® is a bead-formed agarose-based gel filtration matrix. Sepharose® is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose® 2B, Sepharose® 4B and Sepharose® 6B respectively, Both Sepharose® and Sepharose® CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose® CL gels are cross-linked derivatives of Sepharose® 2B, Sepharose® 4B and Sepharose® 6B. The cross-linked form of Sepharose® is chemically and physically more resistant than Sepharose® itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose® gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

Features and Benefits

  • 6% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

Other Notes

Beaded agarose for fractionating molecules of high molecular weight. Cross-linked beaded agarose is more resistant to denaturing conditions, and thus offers more versatility in the choice of sample buffer and eluent. The approximate % agarose concentration is indicated by the product name (Ultrogel A%).

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit

Legal Information

Sepharose is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

Certificate of Origin

Sieving by agarose gels and its use during pulsed-field electrophoresis.
P Serwer
Biotechnology & genetic engineering reviews, 8, 319-343 (1990-01-01)
The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds...
Andrea Trochine et al.
Experimental parasitology, 140, 33-38 (2014-03-19)
Benznidazole (Bzn) is a nitroimidazole drug currently used as first line treatment against Chagas disease, a neglected tropical disease caused by the flagellated protozoan Trypanosoma cruzi. Although the drug has been used since the late 1960s, its mechanism of action...
P Upcroft et al.
Journal of chromatography, 618(1-2), 79-93 (1993-08-25)
Agarose as a medium for separation of DNA was first introduced in 1962 and since the early 1970s agarose submarine gel electrophoresis has been synonymous with separations of DNA molecules larger than 1 kilobase pair (kb). The large pore size...

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