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GE17-0402-01

HiTrap® Protein A High Performance

Cytiva 17-0402-01, pack of 5 × 1 mL

NACRES:
NA.56

ligand

native protein A

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0402-01

storage condition

(20% Ethanol)

parameter

flow rate
<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)

working range

3-9

storage temp.

2-8°C

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GE17-0403-01GE17-0403-03GE17-0402-03
matrix

highly cross-linked 6% agarose

matrix

highly cross-linked 6% agarose

matrix

-

matrix

-

ligand

native protein A

ligand

native protein A

ligand

native protein A

ligand

native protein A

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 17-0402-01

manufacturer/tradename

Cytiva 17-0403-01

manufacturer/tradename

Cytiva 17-0403-03

manufacturer/tradename

Cytiva 17-0402-03

storage condition

(20% Ethanol)

storage condition

(20% Ethanol)

storage condition

(20% Ethanol)

storage condition

(20% Ethanol)

General description

HiTrap® Protein A HP is a prepacked Protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

Features and Benefits

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

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MMYOMAG-74K-13

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Protocols

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Performing a Separation with Cytiva Products Based on Protein A

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Buffer Exchange and Desalting for Affinity Chromatography

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See All

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