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Product Name
HiTrap® Protein A High Performance, Cytiva 17-0403-03, pack of 5 × 5 mL
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0403-03
storage condition
(20% Ethanol)
parameter
0.5 Mpa max. pressure
20 mL/min max. flow rate
5 mL/min flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
size
matrix
cross-linked agarose (spherical)
matrix active group
Protein A
avg. part. size
34 μm
cleaning
2-10
working range
3-9
capacity
~20 mg binding capacity (human IgG / mL resin)()
separation technique
affinity
storage temp.
2-8°C
Related Categories
1 of 4
This Item | GE17-0402-01 | GE17-0403-01 | GE29-0485-76 |
|---|---|---|---|
| parameter 42 psi | parameter flow rate, <4 mL/min flow rate, 42 psi | parameter flow rate, <20 mL/min flow rate, 42 psi | parameter - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition (20% Ethanol) | storage condition (20% Ethanol) | storage condition (20% Ethanol) | storage condition - |
| packaging pack of 5 × 5 mL | packaging pack of 5 × 1 mL | packaging pack of 1 × 5 mL | packaging pack of 1 mL |
| manufacturer/tradename Cytiva 17-0403-03 | manufacturer/tradename Cytiva 17-0402-01 | manufacturer/tradename Cytiva 17-0403-01 | manufacturer/tradename Cytiva 29-0485-76 |
| ligand native protein A | ligand native protein A | ligand native protein A | ligand - |
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Analysis Note
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
General description
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.
Other Notes
Preparation Note
related product
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Articles
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Protocols
This page shows how to prepare samples for purification with affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
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