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GE17-0412-01

HiTrap® Blue High Performance

Cytiva 17-0412-01, pack of 5 × 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

Cibacron Blue F3G-A

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-0412-01

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

size

avg. part. size

34 μm

cleaning

3-13

working range

4-12

General description

HiTrap® Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

Application

Blue Sepharose High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

Features and Benefits

  • Can be used for depletion of albumin from serum.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
  • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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