GE17-0412-01

HiTrap^® Blue High Performance

Cytiva 17-0412-01, pack of 5 × 1 mL

• Synonym(s): Cibacron Blue F3G-A column, albumin affinity column, chromatography column, interferon affinity column, prepacked column
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: Cibacron Blue F3G-A
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 17-0412-01
• parameter: 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• avg. part. size: 34 μm
• cleaning: 3-13
• working range: 4-12

Description

General description

HiTrap^® Blue HP is prepacked with Blue Sepharose^™ High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

Application

Blue Sepharose^™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

Features and Benefits

• Can be used for depletion of albumin from serum.
• Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
• The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids