Documents
GE17-0618-01
Protein G Sepharose™ 4 Fast Flow
Cytiva 17-0618-01, pack of 5 mL
Recommended Products
ligand
recombinant protein G lacking albumin-binding region
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-0618-01
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
average diameter
90 μm (d50v)
cleaning in place
2-10
working range
3-9
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
1 of 4
This Item | GE17-0618-02 | GE17-0618-05 | GE17-1279-01 |
|---|---|---|---|
| packaging pack of 5 mL | packaging pack of 25 mL | packaging pack of 200 mL | packaging pack of 5 mL |
| storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition (20% Ehtanol) |
| matrix 4% cross-linked agarose | matrix 4% cross-linked agarose | matrix 4% cross-linked agarose | matrix 4% cross-linked agarose |
| suitability suitable for bioprocess medium | suitability suitable for bioprocess medium | suitability suitable for bioprocess medium | suitability suitable for bioprocess medium |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
Protein G Sepharose™ 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose™ 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose™ 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.
As member of the BioProcess media range, Protein G Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Binding specificities that complement Protein A media.
- Binds a broad range of IgG species and subclasses.
- Multi-point attachment minimizes ligand leakage.
- Used in a range of research applications.
Analysis Note
Legal Information
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Articles
This page shows a comparison of the relative binding strengths of protein G and protein A to different immunoglobulins.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
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Protocols
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
Related Content
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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