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packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0756-05
storage condition
(20% Ethanol )
parameter
<100 cm/hr flow rate (Sample loading)
100—300 cm/hr flow rate (wash and elution)
technique(s)
liquid chromatography (LC): suitable
matrix
4% agarose
matrix active group
Glutathione
average diameter
90 μm
cleaning in place
4-13
working range
4-13
capacity
>25 mg binding capacity (recombinant GST / ml)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
Related Categories
1 of 4
This Item | GE17-0756-01 | GE17-0756-04 | GE17-5132-02 |
|---|---|---|---|
| matrix 4% agarose | matrix 4% agarose | matrix 4% agarose | matrix highly cross-linked 4% agarose |
| particle size 45-165 μm | particle size 45-165 μm | particle size 45-165 μm | particle size - |
| capacity >25 mg binding capacity (horse liver GST/ml medium) | capacity >25 mg binding capacity (horse liver GST/ml medium) | capacity >25 mg binding capacity (horse liver GST/ml medium) | capacity >10 mg/mL binding capacity |
| manufacturer/tradename Cytiva 17-0756-05 | manufacturer/tradename Cytiva 17-0756-01 | manufacturer/tradename Cytiva 17-0756-04 | manufacturer/tradename Cytiva 17-5132-02 |
| packaging pack of 100 mL | packaging pack of 10 mL | packaging pack of 300 mL | packaging pack of 100 mL |
| ligand glutathione | ligand glutathione | ligand glutathione | ligand glutathione and 10-carbon linker arm |
General description
GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.
Features and Benefits
- High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
- Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Protocols
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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