GE17-0780-01
Protein A-Sepharose™ CL-4B
Cytiva 17-0780-01, pack of 1.5 g
Synonym(s):
Protein A Sepharose, Sepharose CL-4B
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$805.00
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About This Item
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$805.00
ligand
native protein A
packaging
pack of 1.5 g
manufacturer/tradename
Cytiva 17-0780-01
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-10
working range
3-9
capacity
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
storage temp.
2-8°C
Related Categories
1 of 4
This Item | GE17-0963-02 | GE17-0963-03 | GE17-0810-01 |
|---|---|---|---|
| matrix 4% cross-linked agarose | matrix 4% cross-linked agarose | matrix 4% cross-linked agarose | matrix 4% cross-linked agarose |
| particle size 45-165 μm | particle size 45-165 μm | particle size 45-165 μm | particle size 45-165 μm |
| capacity ~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) | capacity ~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) | capacity ~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) | capacity binding capacity(HSA (Mr 68 000) 15-20 mg/ml drained medium, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained medium) |
| packaging pack of 1.5 g | packaging pack of 500 mL | packaging pack of 25 mL | packaging pack of 200 mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition - |
General description
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Features and Benefits
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Storage and Stability
Analysis Note
Legal Information
Storage Class
13 - Non Combustible Solids
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