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NHS Act Sepharose® 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL


shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 25 mL


Cytiva 17-0906-01

storage condition

(100% Isopropanol)


4% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-13(Ligand dependent)

ligand dependent


working range

3-12(3 (ligand dependent)-12 (ligand dependent))


suitable for bioprocess medium

storage temp.


General description

NHS-activated Sepharose® 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose® 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose® 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose® 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

Analysis Note

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Legal Information

Sepharose is a registered trademark of Cytiva


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Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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