GE17-0920-07
IMAC Sepharose™ High Performance
Cytiva 17-0920-07, pack of 100 mL
Synonym(s):
FPLC column packing, IMAC matrix, affinity resin, immobilized metal affinity chromatography polymer, stationary phase, uncharged IMAC resin
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packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0920-07
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Related Categories
1 of 4
This Item | GE17-5268-01 | GE17-5268-02 | GE17-0920-03 |
|---|---|---|---|
| matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose |
| capacity ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) | capacity ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.) | capacity ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.) | capacity ≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.) |
| manufacturer/tradename Cytiva 17-0920-07 | manufacturer/tradename Cytiva 17-5268-01 | manufacturer/tradename Cytiva 17-5268-02 | manufacturer/tradename Cytiva 17-0920-03 |
| packaging pack of 100 mL | packaging pack of 25 mL | packaging pack of 100 mL | packaging pack of 5 × 1 mL |
| avg. part. size 34 μm | avg. part. size 34 μm | avg. part. size 34 μm | avg. part. size 34 μm |
| cleaning in place 2-14(Medium uncharged with metal ions.) | cleaning in place 2-14(Ni2+-stripped medium.) | cleaning in place 2-14(Ni2+-stripped medium.) | cleaning in place - |
General description
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
Preparation Note
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
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Warning
hcodes
Storage Class
3 - Flammable liquids
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Articles
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
Protocols
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
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