Documents

GE17-1196-01

Sigma-Aldrich

HiPrep 26/60 Sephacryl® S-300 HR

Cytiva 17-1196-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

NACRES:
SB.52

material

polypropylene (PP)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

10000-1500000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1196-01

parameter

<2.6 mL/min flow rate
0.15 Mpa (22 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

sample volume

<13 mL

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

working range

3-11

cleaning

2 - 13

separation technique

size exclusion (SEC)

Related Categories

General description

HiPrep 26/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

Suitability

for using preparative separation of biomolecules of up to 13 mL sample volume
Gel filtration columns for low to medium pressure ÄKTA systems.
HiPrep 26/60 Sephacryl S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-300 is used for separating midsize proteins including antibodies and serum proteins.
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Protocols
This page shows how to refold proteins using gel filtration and products from Cytiva.
Read More
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Read More
Related Content
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
Read More

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