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GE17-3712-05

HisTrap Excel

Cytiva 17-3712-05, pack of 5 × 1 mL

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NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-3712-05

parameter

1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
5 bar

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

average diameter

90 μm

cleaning

2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)

working range

2-13

capacity

≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

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packaging

pack of 5 × 1 mL

packaging

pack of 1 mL

packaging

pack of 5 × 5 mL

packaging

pack of 1 mL

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 17-3712-05

manufacturer/tradename

Cytiva 29-0485-86

manufacturer/tradename

Cytiva 17-3712-06

manufacturer/tradename

Cytiva 29-0510-21

parameter

1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)

parameter

1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.), 5 bar

parameter

5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.), 5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)

parameter

flow rate, 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature), 42 psi (H2O at room temperature.)

bed size

7 mm × 25 mm

bed size

7 mm × 25 mm

bed size

16 mm × 25 mm

bed size

7 mm × 25 mm

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
HisTrap is a trademark of Cytiva

pictograms

Environment

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


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Articles

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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