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HisTrap Excel

Cytiva 17-3712-06, pack of 5 × 5 mL


shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 5 × 5 mL


Cytiva 17-3712-06


5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.)
5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm


highly cross-linked 6% agarose

average diameter

90 μm


2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)

working range



≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

General description

HisTrap excel columns are prepacked with Ni Sepharose® excel affinity media for capture and purification of histidine-tagged proteins secreted by immobilized metal ion affinity chromatography (IMAC). The media is resistant to chelating and reducing agents making the medium an excellent choice for the purification of excreted proteins.


Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose® excel medium resistant to chelating and reducing agents

Features and Benefits

  • HiTrap® columns prepacked with Ni Sepharose® excel medium, resistant to chelating and reducing agents
    • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • HisTrap excel columns allow direct purification of cell-free, unclarified samples.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
HiTrap is a registered trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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