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pack of 5 × 5 mL
5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.)
5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)
16 mm × 25 mm
highly cross-linked 6% agarose
2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
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