HiPrep 26/10 Desalting

Cytiva, 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Pricing and availability is not currently available.


polypropylene (PP)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

1000-5000 Da (globular proteins)


pack of 4

mfr. no.

Cytiva, 17-5087-02

storage condition

dry media


<31 mL/min flow rate
0.15 Mpa (22 psi)

bed volume

53 mL

column L × I.D.

10 cm × 26 mm

sample volume

<15 mL


cross-linked dextran

matrix active group

dextran-epichlorohydrin phase

particle size

17-132 μm (dry)


2 - 13

working range

2 - 13

separation technique

size exclusion (SEC)

Related Categories

General description

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Features and Benefits

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.


All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva


NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.
Read More
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Read More
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Read More
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Read More
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
Read More
General instructions for affinity purification using HiTrap columns from Cytiva.
Read More
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Read More
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.