GE17-5319-01
HisTrap™ Fast Flow
Cytiva 17-5319-01, pack of 5 × 1 mL
About This Item
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packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5319-01
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
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This Item | GE17-5255-01 | GE28-9365-51 | GE17-5286-01 |
|---|---|---|---|
| particle size 45-165 μm | particle size 45-165 μm | particle size 45-165 μm | particle size 45-165 μm |
| parameter <20 mL/min flow rate, 42 psi | parameter <4 mL/min flow rate, 42 psi | parameter 1.5 bar (22 psi) (Over the Packed Bed During Operation) | parameter <20 mL/min flow rate, 42 psi (H2O at room temperature.) |
| matrix 6% cross-linked agarose | matrix 6% cross-linked agarose | matrix 6% cross-linked agarose | matrix 6% cross-linked agarose |
| capacity ~40 mg binding capacity(histidine-tagged protein) | capacity ~40 mg binding capacity(histidine-tagged protein) | capacity ~40 mg binding capacity(histidine-tagged protein) | capacity ~40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.), ~40 mg binding capacity(histidine-tagged protein) |
| packaging pack of 5 × 1 mL | packaging pack of 5 × 5 mL | packaging pkg of 1 ea | packaging pack of 5 × 5 mL |
| manufacturer/tradename Cytiva 17-5319-01 | manufacturer/tradename Cytiva 17-5255-01 | manufacturer/tradename Cytiva 28-9365-51 | manufacturer/tradename Cytiva 17-5286-01 |
General description
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Features and Benefits
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Storage and Stability
Analysis Note
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Storage Class
3 - Flammable liquids
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