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GE27-0846-01

Thrombin Protease

Cytiva 27-0846-01

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NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 500 units

manufacturer/tradename

Cytiva 27-0846-01

shipped in

dry ice

storage temp.

−20°C

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This Item
10602400001605204-M605197
vibrant-m

GE27-0846-01

Thrombin Protease

vibrant-m

10602400001

Thrombin

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

storage temp.

−70°C

manufacturer/tradename

Cytiva 27-0846-01

manufacturer/tradename

Roche

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

packaging

pkg of 500 units

packaging

pkg of 20 U

packaging

-

packaging

-

General description

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma.

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors (see pGEX Vectors (GST Gene Fusion System)) containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Application

Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Features and Benefits

  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

signalword

Danger-Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_c

Not applicable


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Articles

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

See All

Protocols

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service