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$376.00
$376.00
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material
resin (Sephadex G-25 DNA grade F)
sterility
non-sterile
manufacturer/tradename
Cytiva 27-5325-01
packaging
pkg of 50 columns
working volume
10 —100 μL
particle size
20-80 μm (dry)
pore size
5000 Da MWCO
working range
2-13
1 of 4
This Item | GE27-5330-01 | GE27-5330-02 | GE27-3565-01 |
|---|---|---|---|
| manufacturer/tradename Cytiva 27-5325-01 | manufacturer/tradename Cytiva 27-5330-01 | manufacturer/tradename Cytiva 27-5330-02 | manufacturer/tradename Cytiva 27-3565-01 |
| packaging pkg of 50 columns | packaging pack of 50 columns | packaging pack of 250 columns | packaging pack of 100 pieces |
| particle size 20-80 μm (dry) | particle size 50-150 μm (dry) | particle size 50-150 μm (dry) | particle size - |
| working range 2-13 | working range - | working range - | working range - |
General description
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
Features and Benefits
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
Preparation Note
Analysis Note
Legal Information
Storage Class
12 - Non Combustible Liquids
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