Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE28-9034-70

GFX PCR DNA and Gel Band Purification Kit

Cytiva 28-9034-70

Sign Into View Organizational & Contract Pricing

UNSPSC Code:
41105501
NACRES:
NA.52

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 100 ea

manufacturer/tradename

Cytiva 28-9034-70

Related Categories

General description

Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg.

illustra GFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.

illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.

Features and Benefits

  • For the isolation and concentration of DNA fragments (50 bp to 40 kb) from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
  • Fast and easy-to-use method with less than 10 min hands-on time.
  • Flexible 10 to 50 μ elution volume for different DNA concentration needs.
  • Improved column design with increased maximum volume, holding of up to 800 μ liquid volume (950 μ without a cap).
  • Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.
  • Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery.
  • Two choices of elution buffer provided to fit downstream applications.
  • Color-coded caps for ease of use.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GFX is a trademark of Cytiva
illustra is a trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ming Liu et al.
AMB Express, 7(1), 58-58 (2017-03-10)
Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical properties of the produced fibre/epoxy composites. For field retting
Sri Nisha Jagathrakshakan et al.
European journal of dentistry, 9(1), 127-132 (2015-02-26)
To identify the prevalence of acidogenic and nonacidogenic bacteria in patients with polycaries lesions, and to ascertain caries specific bacterial prevalence in relation to noncaries controls. Total genomic DNA extracted from saliva of three adults and four children from the
Laura Duran Suja et al.
Frontiers in microbiology, 10, 553-553 (2019-04-06)
A notable feature of the Deepwater Horizon oil spill was the unprecedented formation of marine oil snow (MOS) that was observed in large quantities floating on the sea surface and that subsequently sedimented to the seafloor. Whilst the physical and

Articles

This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

Protocols

DNA cleanup using illustra™ GFX kits from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service