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form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-9187-80
storage condition
(20% ethanol)
parameter
0.3 Mpa max. pressure
20 mL/min max. flow rate
5 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
highly cross-linked 6% agarose
matrix active group
dextrin
particle size
~34 μm
cleaning
2-13
operating pH
>7
capacity
~16 mg binding capacity (MBP2*-b-galactosidase / mL medium)
~7 mg binding capacity (MBP2*-paramyosin-d-Sal / mL medium)
separation technique
affinity
storage temp.
2-8°C
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1 of 4
This Item | GE29-0486-41 | GE28-9187-79 | GE17-5282-01 |
|---|---|---|---|
| parameter <20 mL/min flow rate, 42 psi | parameter 42 psi | parameter <20 mL/min flow rate, 42 psi | parameter flow rate, <20 mL/min flow rate, 42 psi (H2O at room temperature.) |
| matrix highly cross-linked 6% agarose | matrix - | matrix highly cross-linked 6% agarose | matrix highly cross-linked 6% agarose |
| capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity >7 mg/mL binding capacity (medium) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| packaging pack of 5 × 5 mL | packaging pack of 1 mL | packaging pack of 1 × 5 mL | packaging pack of 1 × 5 mL |
| manufacturer/tradename Cytiva 28-9187-80 | manufacturer/tradename Cytiva 29-0486-41 | manufacturer/tradename Cytiva 28-9187-79 | manufacturer/tradename Cytiva 17-5282-01 |
General description
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Features and Benefits
- HiTrap® columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
Preparation Note
Analysis Note
Other Notes
Legal Information
signalword
WarningDanger
hcodes
Hazard Classifications
Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
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Articles
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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