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GE28-9269-85

HiScreen Butyl-S Fast Flow

Cytiva 28-9269-85

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

butyl-S

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9269-85

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

General description

HiScreen Butyl S-FF columns are prepacked with Butyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) resins. The columns are an excellent choice for method optimization and parameter screening.

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 2NA cm.

Media prepacked in HiScreen HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

  • Butyl-S-Sepharose 6 Fast Flow is the least hydrophobic medium in the Sepharose Fast Flow HIC range and particularly useful during the initial stages of a purification process to remove the bulk of impurities.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 1NA cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 2NA cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 0.01 M NaOH)
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva

pictograms

Flame

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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