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GE28-9356-04

Sigma-Aldrich

HiPrep 16/60 Sephacryl® S-400 HR

Cytiva 28-9356-04, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene (PP)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

20000-8000000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9356-04

parameter

<0.5 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

Related Categories

General description

HiPrep 16/60 Sephacryl® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.

Application

Sephacryl® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.

Features and Benefits

  • Gel filtration columns for low to medium pressure AKTA systems.
  • The HiPrep Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.
  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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