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GE28-9893-33

Sigma-Aldrich

HiLoad® 16/600 Superdex® 75 pg

Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

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NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (reinforced)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

3000-70000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-33

parameter

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

agarose-dextran composite
dextran-agarose particle platform
polymeric

matrix active group

dextran-agarose phase
dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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GE28-9893-34GE28-9893-35GE28-9893-36
packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

packaging

pkg of 1 ea

matrix

agarose-dextran composite, dextran-agarose particle platform, polymeric

matrix

agarose dextran composite, dextran-agarose particle platform, polymeric

matrix

agarose dextran composite, dextran-agarose particle platform, polymeric

matrix

agarose-dextran composite, dextran-agarose particle platform, polymeric

matrix active group

dextran-agarose phase

matrix active group

dextran-agarose phase

matrix active group

dextran-agarose phase

matrix active group

dextran-agarose phase

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

General description

HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

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Articles

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from Cytiva.

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Related Content

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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