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$131.00
$131.00
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Product Name
MBPTrap™ HP, Cytiva 29-0486-41, pack of 1 mL
form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0486-41
storage condition
(20% ethanol)
parameter
0.3 Mpa max. pressure
1 mL/min flow rate
4 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
size
matrix
highly cross-linked 6% agarose
matrix active group
dextrin
avg. part. size
34 μm
cleaning
2-13
operating pH
>7
capacity
~16 mg binding capacity (MBP2*-b-galactosidase / mL medium)
~7 mg binding capacity (MBP2*-paramyosin-d-Sal / mL medium)
separation technique
affinity
storage temp.
2-8°C
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Related Categories
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This Item | GE28-9187-80 | GE28-9187-79 | GE17-5282-01 |
|---|---|---|---|
| parameter 42 psi | parameter <20 mL/min flow rate, 42 psi | parameter <20 mL/min flow rate, 42 psi | parameter flow rate, <20 mL/min flow rate, 42 psi (H2O at room temperature.) |
| capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium) | capacity >7 mg/mL binding capacity (medium) |
| storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition (20% Ehtanol) | storage condition - |
| manufacturer/tradename Cytiva 29-0486-41 | manufacturer/tradename Cytiva 28-9187-80 | manufacturer/tradename Cytiva 28-9187-79 | manufacturer/tradename Cytiva 17-5282-01 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| packaging pack of 1 mL | packaging pack of 5 × 5 mL | packaging pack of 1 × 5 mL | packaging pack of 1 × 5 mL |
Features and Benefits
- HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
General description
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Articles
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
Protocols
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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