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GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

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Synonym(s):
Superose® 6 Increase
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superose®

mol wt

5000-5,000,000 Da (globular proteins)

packaging

package of 1 ea

manufacturer/tradename

Cytiva 29-0915-96

parameter

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
25-500 μL sample volume
3 Mpa

bed volume

24 mL

matrix

cross-linked agarose
polymeric

matrix active group

agarose phase

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

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This Item
GE29-0915-98GE29323952GE29321903
Sigma-Aldrich

Sigma-Aldrich

GE29-0915-96

Superose® 6 Increase 10/300 GL

Sigma-Aldrich

Sigma-Aldrich

GE29-0915-98

Superose® 6 Increase

Superose&#174; 6 Prep Grade HiLoad&#174; column thread (16/600), Cytiva 29323952

GE29323952

Superose® 6 Prep Grade HiLoad®

Superose&#174; 6 Increase HiScale&#174; column thread × L (16/40) (40&#160;cm), Cytiva 29321903

GE29321903

Superose® 6 Increase HiScale®

product line

Superose®

product line

Superose®

product line

-

product line

-

packaging

package of 1 ea

packaging

-

packaging

-

packaging

-

matrix

cross-linked agarose, polymeric

matrix

cross-linked agarose, polymeric

matrix

-

matrix

-

matrix active group

agarose phase

matrix active group

agarose phase

matrix active group

-

matrix active group

-

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

-

separation technique

-

General description

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

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Anja Fux et al.
Cell chemical biology, 26(1), 48-59 (2018-11-13)
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Related Content

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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