GS500

Giemsa stain, modified

• Synonym(s): Giemsa Solution
• MDL number: MFCD00081642
• PubChem Substance ID: 24895392
• NACRES: NA.47

Properties

• form: solution
• Quality Level: 500
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• concentration: 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers
• pH: 6.75-6.95
• application(s): hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChI key: NALREUIWICQLPS-UHFFFAOYSA-N

Description

General description

Giemsa stain is significantly been used to identify malarial parasite. It is is majorly employed in chromatin and nuclear membrane staining, including cytoplasm of different cell types. Giemsa stain is most prefered for staining azurophil granules over red cells and neutrophils. It also precisely stains inclusion bodies present in the smear.

Application

Giemsa stain, modified has been used in:

• The Boyden chamber assay to study leukocyte chemotaxis.
• Determining the number of chromosomes present in cells of newly synthesized skin tissue.
• Chromosome aberration test.

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Packaging

500 mL

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H301 + H311 + H331 - H370
• Precautionary Statements: P210 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
• Target Organs: Eyes
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 51.8 °F - closed cup
• Flash Point(C): 11 °C - closed cup