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GS500

Sigma-Aldrich

Giemsa stain, modified

Synonym(s):
Giemsa Solution
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

solution

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

concentration

0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers

pH

6.75-6.95

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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General description

Giemsa stain is significantly been used to identify malarial parasite. It is is majorly employed in chromatin and nuclear membrane staining, including cytoplasm of different cell types. Giemsa stain is most prefered for staining azurophil granules over red cells and neutrophils. It also precisely stains inclusion bodies present in the smear.

Application

Giemsa stain, modified has been used in:
  • The Boyden chamber assay to study leukocyte chemotaxis.
  • Determining the number of chromosomes present in cells of newly synthesized skin tissue.
  • Chromosome aberration test.
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup

Certificate of Analysis

Certificate of Origin

Jing Zhang et al.
Cell death & disease, 11(7), 602-602 (2020-08-01)
Glioblastoma multiforme (GBM) is the most malignant primary brain tumor and has the highest mortality rate among cancers and high resistance to radiation and cytotoxic chemotherapy. Although some targeted therapies can partially inhibit oncogenic mutation-driven proliferation of GBM cells, therapies
The Giemsa stain: its history and applications
Barcia, et al,
International Journal of Surgical Pathology, 15(3) (2007)
Cell Migration (2005)
Histophatologic Techniques (2006)
The effect of gelatin--chondroitin sulfate--hyaluronic acid skin substitute on wound healing in SCID mice
Wang TW, et al.
Biomaterials, 27(33) (2006)

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