There is no official procedure for destaining slides stained with Giemsa or similar stains. However, a common procedure for destaining slides stained with histological dyes is to soak the slides in 70% alcohol with up to 1% HCL or acetic acid. Other suggestions include soaking in xylene or using methanol or a combination of methanol and acid. Once destained, the staining observed may not be optimal, and for best results, freshly prepared slides typically show the best color contrast.
GS500
Giemsa Stain, Modified
Synonym(s):
Giemsa Solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 mL | Check Cart for Availability | $106.00 |
About This Item
form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers
technique(s)
microbe id | staining: suitable
pH
6.75-6.95
application(s)
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
Application
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
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This Item | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) - |
| concentration 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers | concentration 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers | concentration 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers | concentration 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| form solution | form solution | form solution | form solution |
| shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
51.8 °F - closed cup
flash_point_c
11 °C - closed cup
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| GS500-500ML | 04061838261823 |
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What is the process for destaining smears or slides that have been stained with Wright or Giemsa stain?
1 answer-
Helpful?
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Please provide a product comparison for Giemsa solutions GS500 and R03055?
1 answer-
Both products, GS500 and R03055, are intended for staining blood cells and bone marrow cells. The products are slightly different in their formulation and staining properties. It's important to review the instructions for use for each product to determine the best fit for your specific requirements.
Helpful?
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