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GS52

Sigma-Aldrich

GST T2-2, Recombinant Human

Synonym(s):
glutathione S-transferase theta 2
NACRES:
NA.26

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

frozen liquid

specific activity

12.6 units/mg protein (as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T2-2 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.)

mol wt

25 kDa

concentration

1.91 mg/mL

storage temp.

−70°C

Gene Information

human ... GSTT2(2953)

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This Item
GS90GS66GS65
recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

specific activity

12.6 units/mg protein (as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T2-2 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.)

specific activity

8.8 units/mg protein (as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.)

specific activity

93.3 units/mg protein (using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

specific activity

97.96 units/mg protein (using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

mol wt

25 kDa

mol wt

25 kDa

mol wt

26 kDa

mol wt

26 kDa

General description

Glutathione S-transferase theta 2 (GSTT2) is an enzyme that in humans is encoded by the GSTT2 gene. GSTT2 is mapped to human chromosome 22q11.2.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, κ, μ, ω, π, σ, θ and ζ. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
The θ class includes GSTT1, GSTT2, and GSTT2B. GSTT2 and GSTT2B are nearly identical to each other, and share 55% amino acid identity with GSTT1. All three genes may play a role in human carcinogenesis. The GSTT2 gene is a pseudogene in some populations.

Storage and Stability

The enzyme should be used by the end-user customer within 1 year of receipt.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Glutathione S-transferases: an overview in cancer research.
Di Pietro G, et al.
Expert Opinion on Drug Metabolism & Toxicology, 6(2), 153-170 (2010)
Glutathione transferases: substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases.
Allocati N, et al.
Oncogenesis, 7(1), 8-8 (2018)
Molecular cloning of a cDNA and chromosomal localization of a human theta-class glutathione S-transferase gene (GSTT2) to chromosome 22.
Tan K L, et al.
Genomics, 25(2), 381-387 (1995)
Glutathione transferases.
Hayes J D, et al.
Annual Review of Pharmacology and Toxicology, 45, 51-88 (2005)
M W Butler et al.
The European respiratory journal, 38(1), 15-28 (2011-02-26)
The glutathione S-transferase (GST) enzymes catalyse the conjugation of xenobiotics to glutathione. Based on reports that inherited copy number variations (CNVs) modulate some GST gene expression levels, and that the small airway epithelium (SAE) and alveolar macrophages (AMs) are involved

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